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Bcl-2 antibody

Orthogonal
Anti-Bcl-2 antibody used in Western Blot (WB). GTX50413
Anti-Bcl-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50413
Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50413

Cat. No. GTX50413

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Bcl-2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa. 54~58 (G-H-T-P-H) derived from human Bcl-2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165138

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 apoptosis regulator , Bcl-2 , PPP1R50

Cellular Localization

Mitochondrion outer membrane,Endoplasmic reticulum membrane

Background

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Anti-Bcl-2 antibody used in Western Blot (WB). GTX50413

GTX50413 WB Image

Orthogonal

WB analysis of extracts from HeLa, A2780, and 293 cells using GTX50413 Bcl-2 antibody.

Anti-Bcl-2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50413

GTX50413 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50413 Bcl-2 antibody.

Anti-Bcl-2 antibody used in IHC (Paraffin sections) (IHC-P). GTX50413

GTX50413 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50413 Bcl-2 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

Application Reference

Zhao X et al. Cancer Cell Int 2021; 21 (1) : 644 Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells.
Application : WB
Reactivity : Human
Sharikova AV et al. J Neuroimmune Pharmacol 2018; (Epub)
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399