GeneTex
United States (US)

Bmi1 antibody

KO/KD
GTX114008_40150_IHC_18121410_469.jpg
GTX114008_40150_IHC_2_18121410_953.jpg
GTX114008_40150_WB_2_18121410_559.jpg
GTX114008_40150_IFA_18121410_146.jpg
GTX114008_40150_IP_18121410_898.jpg
GTX114008_40150_WB_M_18121410_135.jpg
GTX114008_40150_WB_18121410_248.jpg
GTX114008_40150_20171117_WB_2_18121410_671.jpg
GTX114008_40150_20180907_WB_shRNA_watermark_18121410_833.jpg
GTX114008_40150_20171117_WB_18121410_954.jpg

Cat No. GTX114008

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

293T , A549 , H1299 , HCT116 , K562 , THP-1 , THP-1 , HL-60 , C8D30 , BCL-1 , Raw264.7

Predict Reactivity

Rat, Bovine, Cat, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLVI2/BMI1 antibody, MGC12685 antibody, PCGF4 antibody, RNF51 antibody, BMI1 antibody, polycomb group protein Bmi1 antibody, ring finger protein 51 antibody, flvi-2/bmi-1 antibody, polycomb complex protein BMI-1 antibody, B lymphoma Mo-MLV insertion region 1 homolog antibody, murine leukemia viral (bmi-1) oncogene homolog antibody, polycomb group RING finger protein 4 antibody, BMI1 polycomb ring finger oncogene antibody

Cellular Localization

Nucleus , Cytoplasm

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Database

Research Area

DATA IMAGES
GTX114008_40150_IHC_18121410_469.jpg

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114008_40150_IHC_2_18121410_953.jpg

GTX114008 IHC-P Image

Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Bmi1 antibody (GTX114008) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114008_40150_WB_2_18121410_559.jpg

GTX114008 WB Image

Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 µg THP-1 whole cell lysate/extract
B. 30 µg HL-60 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008_40150_IFA_18121410_146.jpg

GTX114008 ICC/IF Image

BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min.
Green: BMI1 protein stained by BMI1 antibody (GTX114008) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX114008_40150_IP_18121410_898.jpg

GTX114008 IP Image

Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 μg of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX114008_40150_WB_M_18121410_135.jpg

GTX114008 WB Image

Bmi1 antibody detects BMI1 protein by western blot analysis.
A. 30 µg C8D30 whole cell lysate/extract
B. 30 µg BCL-1 whole cell lysate/extract
C. 30 µg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008_40150_WB_18121410_248.jpg

GTX114008 WB Image

Bmi1 antibody detects Bmi1 protein by western blot analysis.
A. 30 µg K562 whole cell lysate/extract
B. 30 µg THP-1 whole cell lysate/extract
10% SDS-PAGE
Bmi1 antibody (GTX114008) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008_40150_20171117_WB_2_18121410_671.jpg

GTX114008 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008_40150_20180907_WB_shRNA_watermark_18121410_833.jpg

GTX114008 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114008_40150_20171117_WB_18121410_954.jpg

GTX114008 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

Bmi1 antibody Cat No. GTX114008

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 31-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169