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Bora antibody

Anti-Bora antibody used in IHC (Paraffin sections) (IHC-P). GTX85293
Anti-Bora antibody used in IHC (Paraffin sections) (IHC-P). GTX85293
Anti-Bora antibody used in Western Blot (WB). GTX85293

Cat. No. GTX85293

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($409)
See all Bora products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

Jurkat Cell Lysate(GTX27899)

Product Note

This antibody is specific for Bora N-Terminus

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bora antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human Bora.The immunogen is located within amino acids 70 - 120 of Bora.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BORA aurora kinase A activator , C13orf34

Background

Bora (Protein aurora borealis) is a key activator of Aurora Related Protein Kinase A (ARK-1), which is a centrosome-associated serine/threonine kinase that regulates centrosome maturation, bipolar spindle assembly and chromosome segregation during mitosis. Bora is localized to the nucleus until mitosis is initiated, then translocates to the cytoplasm in a Cdc2 dependent manner. Activation of Cdc2 initiates the release of Bora into the cytoplasm where it can bind and activate ARK-1. PLK1 (polo-like kinase-1) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation by ARK-1. Bora and ARK-1 cooperatively activate PLK1 and control mitotic entry. Bora mutants result in multipolar spindles in mitosis identical to those observed when ARK-1 function is blocked. Thus, the ARK1-Bora-PLK1 regulatory circuit in mammalian cells elucidates a key mechanism in cell cycle regulation. At least three isoforms of Bora are known to exist.

Database

Research Area

DATA IMAGES

Anti-Bora antibody used in IHC (Paraffin sections) (IHC-P). GTX85293

GTX85293 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85293 Bora antibody.
Working concentration : 20 μg/ml

Anti-Bora antibody used in IHC (Paraffin sections) (IHC-P). GTX85293

GTX85293 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85293 Bora antibody.
Working concentration : 2.5 μg/ml

Anti-Bora antibody used in Western Blot (WB). GTX85293

GTX85293 WB Image

WB analysis of Jurkat cell lysate using GTX85293 Bora antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409