GeneTex
United States (US)

BrdU antibody [B33.1]

Cat No. GTX27781

Host Mouse
Clonality Monoclonal
Clone Name B33.1
Isotype IgG1
Application IHC-P, IHC-F
APPLICATION

Application Note

Formalin-fixed, paraffin-embedded tissue sections. For a three-stepavidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. We recommendan incubation time and temperature of 30 minutes at 37degC for this antibody.Enzymatic predigestion with proteolytic enzymes is recommended. Subsequently incubate theslides for 45 minutes in 95% formamide in 0.15M trisodium citrate at 70ºC for the partial denaturationof double stranded DNA.

Specificity/Sensitivity

This antibody recognizes BrdU in single stranded DNA, free BrdU or BrdU coupled to protein carrier. The antibody binds to the cells which have incorporated BrdU into their DNA during the S-phase of the cell cycle. It labels BrdU in the nuclei of a wide range of cell types.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Chemical / Small Molecule conjugated to BSA.

Purification

Purified IgG
From hybridoma culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bromodeoxyuridine antibody BUdr antibody

Background

The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information (such as the S-phase transit rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

Research Area

REFERENCE
Package List Price ($)
$ 319