C/EBP alpha antibody [N1], N-term

Name: C/EBP alpha antibody [N1], N-term
Brand: GeneTex
SKU: GTX100674
Price: 399.00 USD
Rating: 4 (2 reviews)

Anti-C/EBP alpha antibody [N1], N-term used in Immunoprecipitation (IP). GTX100674

Anti-C/EBP alpha antibody [N1], N-term used in ChIP assay (ChIP assay). GTX100674

Anti-C/EBP alpha antibody [N1], N-term used in Western Blot (WB). GTX100674

Cat. No. GTX100674

Reference 7

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse, Sheep
Package	100 μl ($399), 25 μl ($169)

See all C/EBP alpha products

View product citations for antibody GTX100674 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

C2C12

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.59 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618812

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein alpha , C/EBP-alpha , CEBP

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq]

Database

Gene ID: 1050 CEBPA
UniProt: P49715 CEBPA

Research Area

DATA IMAGES

GTX100674 IP Image

C/EBP alpha antibody immunoprecipitates C/EBP alpha protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : Control with 3 μg of pre-immune rabbit IgG B : Immunoprecipitation of C/EBP alpha by 3 μg of C/EBP alpha antibody (GTX100674) 10% SDS-PAGE The immunoprecipitated C/EBP alpha protein was detected by C/EBP alpha antibody (GTX100674) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX100674 ChIP assay Image

Cross-linked ChIP was performed with A549 chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to CAMP promoter.
ChIP experiment and primer designs are based on FASEB J. 2005 Jul;19(9):1067-77.

GTX100674 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100674 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [N1], N-term (GTX100674) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX100674 WB Image

The data was published in the journal PLoS One in 2015. PMID: 26121644

REFERENCE

Application Reference

Yang H et al. Anim Biosci 2022; 35 (7) : 1010-1020 Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue.

Application : WB, IHC-P

Reactivity : Sheep

Liu H et al. Diabetes Metab Syndr Obes 2020; 13 : 3075-3087 Liraglutide and Insulin Have Contrary Effects on Adipogenesis of Human Adipose-Derived Stem Cells via Wnt Pathway.

Application : WB

Reactivity : Human

Chen CC et al. Transl Res 2019; (Epub) Pigment epithelium-derived factor inhibits adipogenesis in 3T3-L1 adipocytes and protects against high-fat diet-induced obesity and metabolic disorders in mice.

Application : WB

Reactivity : Mouse

de Kerckhove M et al. EMBO Mol Med 2018; (Epub) Targeting miR-223 in neutrophils enhances the-clearance of Staphylococcus aureus in infected-wounds.

Application : ChIP assay

Wu JS et al. Mol Neurobiol 2017; (Epub) Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPβ-Driven PPAR-γ Transcription.

Application : ChIP assay

Reactivity : Mouse

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