GeneTex

  • Country / Location Selection

United States (US)

C/EBP beta antibody [1H7]

Anti-C/EBP beta antibody [1H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15050

Cat. No. GTX15050

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1H7

Isotype

 IgG

Application

 WB, ICC/IF, IP, ChIP assay, Gel supershift assays

Reactivity

 Mouse, Rat
Package
50 μg ($399)
See all C/EBP beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF 4 μg/ml
IP Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

Product Note

This antibody detects the LAP*, LAP and LIP isoforms of C/EBP beta.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant mouse LIP.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369010

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT/enhancer binding protein (C/EBP), beta , C/EBPbeta , CRP2 , IL-6DBP , LAP , LIP , NF-IL6 , NF-M , Nfil6

Cellular Localization

Nucleus,Cytoplasm

Background

The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues.? These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP.? One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells.? Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation.? From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.

Database

Research Area

DATA IMAGES

Anti-C/EBP beta antibody [1H7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15050

GTX15050 ICC/IF Image

ICC/IF analysis of P388D1 cells using GTX15050 C/EBP beta antibody [1H7].

REFERENCE

Application Reference

H Qi et al. Cell Mol Biol Lett 2023; Activation of BZW1 by CEBPB in macrophages promotes eIF2α phosphorylation-mediated metabolic reprogramming and endoplasmic reticulum stress in MRL/lpr lupus-prone mice
Application : ChIP assay
Thunen A et al. Adipocyte 2022; 11 (1) : 44892 Role of lncRNA LIPE-AS1 in adipogenesis.
Application : WB
Reactivity : Mouse
Wu JS et al. Mol Neurobiol 2017; (Epub) Clinacanthus nutans Mitigates Neuronal Apoptosis and Ischemic Brain Damage Through Augmenting the C/EBPβ-Driven PPAR-γ Transcription.
Application : ChIP assay
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for C/EBP beta antibody [1H7] (GTX15050). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399