GeneTex

  • Country / Location Selection

United States (US)

C/EBP beta antibody

KO/KD Orthogonal
Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100675
Anti-C/EBP beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100675
Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675
Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675
Anti-C/EBP beta antibody used in Immunoprecipitation (IP). GTX100675
Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675
Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675

Cat. No. GTX100675

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay, IHC

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all C/EBP beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949960

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CCAAT enhancer binding protein beta , C/EBP-beta , IL6DBP , NF-IL6 , TCF5

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100675

GTX100675 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using C/EBP beta(GTX100675) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C/EBP beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100675

GTX100675 ICC/IF Image

C/EBP beta antibody detects C/EBP beta protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C/EBP beta stained by C/EBP beta antibody (GTX100675) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675

GTX100675 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP beta antibody (GTX100675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675

GTX100675 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP beta antibody. The precipitated DNA was detected by PCR with primer set targeting to CR2pD.
ChIP experiment and primer designs are based on Mol Immunol. 2009 Aug;46(13):2613-22.

Anti-C/EBP beta antibody used in Immunoprecipitation (IP). GTX100675

GTX100675 IP Image

Immunoprecipitation of CEBPB protein from HeLa nuclear extracts using 5 μg of C/EBP beta antibody (GTX100675).
Western blot analysis was performed using C/EBP beta antibody (GTX100675) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675

GTX100675 WB Image

KO/KD Orthogonal

The data was published in the journal PLoS One in 2015.PMID: 26046540

Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675

GTX100675 ChIP assay Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29875371

REFERENCE

Application Reference

Polcaro G et al. Mol Cancer 2024; 23 (1) : 63 rs822336 binding to C/EBPβ and NFIC modulates induction of PD-L1 expression and predicts anti-PD-1/PD-L1 therapy in advanced NSCLC.
Hartl L et al. Int J Mol Sci 2023; 24 (2) C/EBP-Family Redundancy Determines Patient Survival and Lymph Node Involvement in PDAC.
Application : IHC-P
Reactivity : Human
Zhao H et al. Cell Rep 2022; 38 (4) : 110253 Opioid receptor signaling suppresses leukemia through both catalytic and non-catalytic functions of TET2.
Application : IP
Du L et al. Front Immunol 2021; 12 : 657071 NSP2 Is Important for Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus to Trigger High Fever-Related COX-2-PGE2 Pathway in Pigs.
Application : WB
Reactivity : Human
Bastola S et al. Nat Commun 2020; 11 (1) : 4660 Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy.
Application : ChIP assay
Show More +
Submit a Reference

REVIEW

There are currently no reviews for C/EBP beta antibody (GTX100675). Be the first to share your experience with this product.
Submit a Review
Application Reference
Polcaro G et al. Mol Cancer 2024; 23 (1):63 rs822336 binding to C/EBPβ and NFIC modulates induction of PD-L1 expression and predicts anti-PD-1/PD-L1 therapy in advanced NSCLC.
Hartl L et al. Int J Mol Sci 2023; 24 (2) C/EBP-Family Redundancy Determines Patient Survival and Lymph Node Involvement in PDAC.
Application : IHC-P
Reactivity : Human
Zhao H et al. Cell Rep 2022; 38 (4):110253 Opioid receptor signaling suppresses leukemia through both catalytic and non-catalytic functions of TET2.
Application : IP
Du L et al. Front Immunol 2021; 12:657071 NSP2 Is Important for Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus to Trigger High Fever-Related COX-2-PGE2 Pathway in Pigs.
Application : WB
Reactivity : Human
Bastola S et al. Nat Commun 2020; 11 (1):4660 Glioma-initiating cells at tumor edge gain signals from tumor core cells to promote their malignancy.
Application : ChIP assay
Lin CK et al. Sci Rep 2018; 8 (1):8676 Lobohedleolide suppresses hepatitis C virus replication via JNK/c-Jun-C/EBP-mediated down-regulation of cyclooxygenase-2 expression.
Application : IP
Reactivity : Human
Chen Y et al. PLoS One 2015; 10 (6):e0127957 Downregulation of TNIP1 Expression Leads to Increased Proliferation of Human Keratinocytes and Severer Psoriasis-Like Conditions in an Imiquimod-Induced Mouse Model of Dermatitis.
Application : IHC
Reactivity : Human
Enomoto A et al. Eur J Cancer 2013; 49 (16):3547-58 The HSP90 inhibitor 17-allylamino-17-demethoxygeldanamycin modulates radiosensitivity by downregulating serine/threonine kinase 38 via Sp1 inhibition.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169