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C/EBP beta antibody

Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675
Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100675
Anti-C/EBP beta antibody used in Immunoprecipitation (IP). GTX100675
Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675
Anti-C/EBP beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100675

Cat No. GTX100675

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay, IHC
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CCAAT enhancer binding protein beta,C/EBP-beta,IL6DBP,NF-IL6,TCF5

Cellular Localization

Nucleus

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-C/EBP beta antibody used in ChIP assay (ChIP assay). GTX100675

GTX100675 ChIP assay Image

Cross-linked ChIP was performed with Raji chromatin extract and 5 μg of either control rabbit IgG or anti-C/EBP beta antibody. The precipitated DNA was detected by PCR with primer set targeting to CR2pD.
ChIP experiment and primer designs are based on Mol Immunol. 2009 Aug;46(13):2613-22.

Anti-C/EBP beta antibody used in IHC (Paraffin sections) (IHC-P). GTX100675

GTX100675 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using C/EBP beta(GTX100675) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-C/EBP beta antibody used in Immunoprecipitation (IP). GTX100675

GTX100675 IP Image

Immunoprecipitation of CEBPB protein from HeLa nuclear extracts using 5 μg of C/EBP beta antibody (GTX100675).
Western blot analysis was performed using C/EBP beta antibody (GTX100675).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-C/EBP beta antibody used in Western Blot (WB). GTX100675

GTX100675 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP beta antibody (GTX100675) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
The observed M.W. is based on the publication: PMID: 27626488

Anti-C/EBP beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100675

GTX100675 ICC/IF Image

C/EBP beta antibody detects C/EBP beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C/EBP beta stained by C/EBP beta antibody (GTX100675) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169