Reconstitute to a concentration of 1.0 mg/ml in ice-cold water containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70ºC.
Purity was assessed by SDS-PAGE (≥95%) and by HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Complement Fragments C5a
22 complement C5a is a 77-amino acid peptide generated during complement activation from the alpha -chain of complement C5. 22 C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa 22 C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.