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CAD antibody

Anti-CAD antibody used in Western Blot (WB). GTX28407
Anti-CAD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28407

Cat. No. GTX28407

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($479)
See all DFFB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 10μg/ml
Not tested in other applications.

Calculated MW

243 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide corresponding to aa 203-218 of human DFF40 (accession no. NP_004393).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CAD , CDG1Z , carbamoylphosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase , carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase

Cellular Localization

Cytoplasm,Nucleus

Background

The de novo synthesis of pyrimidine nucleotides is required for mammalian cells to proliferate. This gene encodes a trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis: carbamoylphosphate synthetase (CPS II), aspartate transcarbamoylase, and dihydroorotase. This protein is regulated by the mitogen-activated protein kinase (MAPK) cascade, which indicates a direct link between activation of the MAPK cascade and de novo biosynthesis of pyrimidine nucleotides. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-CAD antibody used in Western Blot (WB). GTX28407

GTX28407 WB Image

Western blot analysis of DFF40 in Jurkat cell lysate with CAD antibody (GTX28407) at (A) 0.5, (B) 1 and (C) 2 g/ml.

Anti-CAD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28407

GTX28407 ICC/IF Image

Immunocytochemical staining of DFF40 in K562 cells with CAD antibody (GTX28407) at 10μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 479