GeneTex
United States (US)

CAD peptide

Cat No. GTX28440

Application Blocking
Species Human
APPLICATION

Application Note

The peptide is used for blocking the activity of DFFB antibody (GTX31262). Incubatingthe peptide with equal volume of antibody for 30 min at 37ºC usually completely blocks the antibody activity in Western blotting.
PROPERTIES

Form

Liquid

Buffer

PBS pH7.2 with 10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl, 0.1% bovine serum albumin, and 0.02% sodium azide

Concentration

0.20mg/ml(Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Caspase activated deoxyribonuclease Caspase activated DNase Caspase activated nuclease CPAN DFF2 DFF40 DFFB DNA fragmentation factor 40 kD beta subunit DNA fragmentation factor 40kDa beta polypeptide

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation ofchromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD, which stands for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Research Area

Package List Price ($)
$ 179