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CALM antibody

Anti-CALM antibody used in Western Blot (WB). GTX119631
Anti-CALM antibody used in Western Blot (WB). GTX119631

Cat. No. GTX119631

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

HepG2 , NIH-3T3

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CALM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170495

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphatidylinositol binding clathrin assembly protein , CALM , CLTH , LAP

Cellular Localization

Membrane , clathrin-coated pit , Golgi apparatus , Cytoplasmic vesicle , clathrin-coated vesicle

Background

Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction.

Database

Research Area

DATA IMAGES

Anti-CALM antibody used in Western Blot (WB). GTX119631

GTX119631 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
7.5% SDS PAGE
GTX119631 diluted at 1:1000

Anti-CALM antibody used in Western Blot (WB). GTX119631

GTX119631 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
7.5% SDS PAGE
GTX119631 diluted at 1:500

REFERENCE

There are currently no references for CALM antibody (GTX119631). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CALM antibody (GTX119631). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169