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CASC5 antibody

Anti-CASC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX66163
Anti-CASC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX66163
Anti-CASC5 antibody used in Western Blot (WB). GTX66163

Cat. No. GTX66163

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all CASC5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

265 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 1-300 of human CASC5 (NP_733468.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

kinetochore scaffold 1 , AF15Q14 , CASC5 , CT29 , D40 , MCPH4 , PPP1R55 , Spc7 , hKNL-1 , hSpc105

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a component of the multiprotein assembly that is required for creation of kinetochore-microtubule attachments and chromosome segregation. The encoded protein functions as a scaffold for proteins that influence the spindle assembly checkpoint during the eukaryotic cell cycle and it interacts with at least five different kinetochore proteins and two checkpoint kinases. In adults, this gene is predominantly expressed in normal testes, various cancer cell lines and primary tumors from other tissues and is ubiquitously expressed in fetal tissues. This gene was originally identified as a fusion partner with the mixed-lineage leukemia (MLL) gene in t(11;15)(q23;q14). Mutations in this gene cause autosomal recessive primary microcephaly-4 (MCPH4). Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-CASC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX66163

GTX66163 IHC-P Image

IHC-P analysis of human colon tissue using GTX66163 CASC5 antibody.
Dilution : 1:100

Anti-CASC5 antibody used in IHC (Paraffin sections) (IHC-P). GTX66163

GTX66163 IHC-P Image

IHC-P analysis of rat testis tissue using GTX66163 CASC5 antibody.
Dilution : 1:100

Anti-CASC5 antibody used in Western Blot (WB). GTX66163

GTX66163 WB Image

WB analysis of various sample lysates using GTX66163 CASC5 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:3000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399