CBP antibody [C3], C-term

Name: CBP antibody [C3], C-term
Brand: GeneTex
SKU: GTX101249
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CBP antibody [C3], C-term used in Western Blot (WB). GTX101249

Anti-CBP antibody [C3], C-term used in Immunoprecipitation (IP). GTX101249

Anti-CBP antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101249

Anti-CBP antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101249

Anti-CBP antibody [C3], C-term used in ChIP assay (ChIP assay). GTX101249

Cat. No. GTX101249

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity	Human, Mouse, Monkey
Package	100 μl ($399), 25 μl ($169)

See all CBP products

View product citations for antibody GTX101249 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IP	1:100-1:500
ChIP assay	Assay dependent

Not tested in other applications.

Calculated MW

265 kDa. ( Note )

Positive Control

A549 , H1299 , HCT-116 , HeLa

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CBP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240560

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CREB binding protein , CBP , KAT3A , MKHK1 , RSTS , RSTS1

Cellular Localization

Nucleus

Background

This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 1387 CREBBP
UniProt: Q92793 CREBBP

Research Area

DATA IMAGES

GTX101249 WB Image

Wild-type (WT) and CBP knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101249 IP Image

CBP antibody immunoprecipitates CBP protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of CBP protein by 2.5 μg of CBP antibody (GTX101249) 12% SDS-PAGE The immunoprecipitated CBP protein was detected by CBP antibody (GTX101249) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101249 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249 ICC/IF Image

CBP antibody [C3], C-term detects CBP protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CBP protein stained by CBP antibody [C3], C-term (GTX101249) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 μm.

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.
Sample: Paraffin-embedded mouse skin.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249 ChIP assay Image

CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of CREBBP antibody (GTX101249) The precipitated DNA was detected by PCR with primer set targeting to snail promoter.

GTX101249 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CBP antibody [C3], C-term (GTX101249) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101249 WB Image

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CBP antibody [C3], C-term (GTX101249) dilution: 1:750.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101249 IHC-P Image

CBP antibody [C3], C-term detects CBP protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
CBP stained by CBP antibody [C3], C-term (GTX101249) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6

REFERENCE

Application Reference

Pratiwi HM et al. Front Cell Dev Biol 2023; 11 : 1170691 Heterochronic development of pelvic fins in zebrafish: possible involvement of temporal regulation of pitx1 expression.

Chen SJ et al. Theranostics 2021; 11 (10) : 4672-4687

Application : WB

Reactivity : Human

Sengupta S et al. DNA Repair (Amst) 2018; 66-67 : 1-10 Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress.

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REVIEW