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CBS antibody [GT519]

KO/KD Overexpression
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777
Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777
Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

Cat. No. GTX628777

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT519

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CBS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

rat liver , Raji , K562 , mouse liver , 293T , HeLa , CBS-transfected 293T

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888050

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystathionine-beta-synthase , CBSL , HIP4

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500.

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

KO/KD

Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

CBS antibody [GT519] detects CBS protein by Western blot analysis.
A. 30 μg Raji whole cell lysate/extract
B. 30 μg K562 whole cell lysate/extract
7.5 % SDS-PAGE
CBS antibody [GT519] (GTX628777) dilution: 1:1000

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777

GTX628777 IHC-P Image

CBS antibody [GT519] detects CBS protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
CBS antibody [GT519] (GTX628777) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBS antibody [GT519] used in IHC (Paraffin sections) (IHC-P). GTX628777

GTX628777 IHC-P Image

CBS antibody [GT519] detects CBS protein at cytoplasm in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CBS antibody [GT519] (GTX628777) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

CBS antibody [GT519] detects CBS protein by Western blot analysis.
A. 50 μg mouse liver lysate/extract
7.5 % SDS-PAGE
CBS antibody [GT519] (GTX628777) dilution: 1:2000

Anti-CBS antibody [GT519] used in Western Blot (WB). GTX628777

GTX628777 WB Image

CBS antibody [GT519] detects CBS protein by Western blot analysis.
A. 50 μg rat liver lysate/extract
7.5 % SDS-PAGE
CBS antibody [GT519] (GTX628777) dilution: 1:1000

REFERENCE

Application Reference

Zheng J et al. Cell Death Dis 2021; 12 (7) : 698 Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines.
Application : WB
Reactivity : Human
Ahmad A et al. Int J Mol Med 2016; (Epub) Effect of endotoxemia in mice genetically deficient in cystathionine-γ-lyase, cystathionine-β-synthase or 3-mercaptopyruvate sulfurtransferase.
Application : WB
Reactivity : Mouse
Pincini A et al. Cell Cycle 2013; 12 (15) : 2409-22 Identification of p130Cas/ErbB2-dependent invasive signatures in transformed mammary epithelial cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations