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CBS antibody

KO/KD Overexpression
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400
Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400
Anti-CBS antibody used in Immunoprecipitation (IP). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400
Anti-CBS antibody used in Western Blot (WB). GTX113400

Cat. No. GTX113400

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CBS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse liver , 293T , HeLa , CBS-transfected 293T

Predict Reactivity

Rabbit, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.81 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CBS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036461

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cystathionine-beta-synthase , CBSL , HIP4

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

KO/KD

Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400

GTX113400 IHC-P Image

CBS antibody detects CBS protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CBS stained by CBS antibody (GTX113400) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CBS antibody used in IHC (Paraffin sections) (IHC-P). GTX113400

GTX113400 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using CBS(GTX113400) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CBS antibody used in Immunoprecipitation (IP). GTX113400

GTX113400 IP Image

CBS antibody immunoprecipitates CBS protein in IP experiments.
IP samples: HepG2 whole cell extract
A. 50 μg HepG2 whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of CBS protein by 4 μg CBS antibody (GTX113400)
7.5 % SDS-PAGE
The immunoprecipitated CBS protein was detected by CBS antibody (GTX113400) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

CBS antibody detects CBS protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113400

GTX113400 ICC/IF Image

CBS antibody detects CBS protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: CBS stained by CBS antibody (GTX113400) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CBS antibody used in Western Blot (WB). GTX113400

GTX113400 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody (GTX113400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wijerathne CUB et al. Biomolecules 2020; 10 (11) Kidney Ischemia-Reperfusion Decreases Hydrogen Sulfide and Increases Oxidative Stress in the Heart.
Application : WB
Reactivity : Rat
Morales-Loredo H et al. Am J Physiol Heart Circ Physiol 2019; 317 (5) : H1157-H1165 Hydrogen sulfide regulation of renal and mesenteric blood flow.
Application : IHC-P
Reactivity : Rat
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169