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CCN3 antibody

Overexpression
Anti-CCN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103377
Anti-CCN3 antibody used in Western Blot (WB). GTX103377
Anti-CCN3 antibody used in Western Blot (WB). GTX103377
Anti-CCN3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103377
Anti-CCN3 antibody used in Western Blot (WB). GTX103377
Anti-CCN3 antibody used in Western Blot (WB). GTX103377

Cat. No. GTX103377

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CCN3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Mouse brain , human CCN3-transfected 293T cells

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CCN3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951010

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cellular communication network factor 3 , IBP-9 , IGFBP-9 , IGFBP9 , NOV , NOVh

Cellular Localization

Secreted

Background

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CCN3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103377

GTX103377 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CCN3 (GTX103377) antibody at 1:200 dilution.

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Sample(30 μg whole cell lysate)
A:293T
12% SDS PAGE
GTX103377 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody (GTX103377) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCN3 antibody used in IHC (Paraffin sections) (IHC-P). GTX103377

GTX103377 IHC-P Image

Immunohistochemical analysis of paraffin-embedded gastric cancer N87 xenograft, using NOV(GTX103377) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody (GTX103377) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CCN3 antibody used in Western Blot (WB). GTX103377

GTX103377 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody (GTX103377) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Poli G et al. FASEB J 2018; : fj201801028RR Human fetal adrenal cells retain age-related stem- and endocrine-differentiation potential in culture.
Application : IHC-P
Reactivity : Human
Shih YC et al. Circ Res 2018; (Epub) Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

CCN3 antibody Cat. No. GTX103377

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-CCN3 antibody used in Western Blot (WB). GTX103377
Date : Anonymous submitted on 03-Jul-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : mice ventricle
Amount used : 30 μg
Blocking : 5% BSA
Primary Antibody : 1:1000, 4°C, 16Hr
Figure Note : ISO: Isoproterenol
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production