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CD2 antibody [4E4]

Overexpression
Anti-CD2 antibody [4E4] used in IHC (Paraffin sections) (IHC-P). GTX84724
Anti-CD2 antibody [4E4] used in Western Blot (WB). GTX84724
Anti-CD2 antibody [4E4] used in Western Blot (WB). GTX84724
Anti-CD2 antibody [4E4] used in Flow cytometry (FACS). GTX84724

Cat. No. GTX84724

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4E4

Isotype

 IgG1

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($519)
See all CD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P 1:500
FACS 1:100
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human CD2 expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD2 molecule , LFA-2 , SRBC , T11

Cellular Localization

Cell membrane

Background

CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Database

Research Area

DATA IMAGES

Anti-CD2 antibody [4E4] used in IHC (Paraffin sections) (IHC-P). GTX84724

GTX84724 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84724 CD2 antibody [4E4].
Antigen retrieval : heat-induced antigen retrieval with 1mM EDTA in 10mM Tris buffer (pH8.5) at 120ºC for 3 min.
Dilution : 1:500

Anti-CD2 antibody [4E4] used in Western Blot (WB). GTX84724

GTX84724 WB Image

WB analysis of various cell lines using GTX84724 CD2 antibody [4E4].
Loading : 35 ug per lane
Dilution : 1:200

Anti-CD2 antibody [4E4] used in Western Blot (WB). GTX84724

GTX84724 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84724 CD2 antibody [4E4].
Loading : 5 ug per lane

Anti-CD2 antibody [4E4] used in Flow cytometry (FACS). GTX84724

GTX84724 FACS Image

FACS analysis of Jurkat cells using GTX84724 CD2 antibody [4E4].
Red : Primary antibody
Black : Isotype control antibody
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519