APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
5-10μg/ml |
Note :
IHC-P
Human Spleen shows nucleolar staining in select splenocytes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89547-PEP).
Predict Reactivity
Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence PKRKVTFQGVGDE-C, from the N Terminus of the protein sequence according to NP_006101.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD2 cytoplasmic tail binding protein 2 , FWP010 , LIN1 , PPP1R59 , Snu40 , U5-52K
Cellular Localization
Cytoplasm , Nucleus
Background
This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined. [provided by RefSeq, Nov 2008]
Database
Research Area
DATA IMAGES
|
GTX89547 WB Image
WB analysis of human thymus lysate using GTX89547 CD2BP2 antibody, N-term. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
|
|
GTX89547 IHC-P Image
IHC-P analysis of human spleen using GTX89547 CD2BP2 antibody, N-term. Antigen retrieval : citrate buffer pH 6 Dilution : 5μg/ml
|
REFERENCE
There are currently no references for CD2BP2 antibody, N-term (GTX89547). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD2BP2 antibody, N-term (GTX89547). Be the first to share your experience with this product.