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CD3 antibody [1F4]

Anti-CD3 antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX76493

Cat. No. GTX76493

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F4

Isotype

 IgM

Application

 WB, IHC-P, IHC-Fr, FACS, IP

Reactivity

 Rat
Package
100 μg ($399)
See all CD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/10
IHC-Fr 1/10-1/25
FACS 1/10-1/25
IP Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Product Note

In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

F344 strain rat T cells stimulated with PMA (TPA) and calcium ionophore

Purification

Ammonium sulphate precipitation
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_379307

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-CD3 antibody [1F4] used in IHC (Paraffin sections) (IHC-P). GTX76493

GTX76493 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX76493 CD3 antibody [1F4].

REFERENCE

Application Reference

Wu F et al. eNeuro 2022; (Epub) Timing of splenectomy after acute spinal cord injury.
Reactivity : Rat
Ciccia A et al. Genes Dev 2009; 23 (20) : 2415-25 The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart.
Application : WB
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399