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CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free)

Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102
Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102
Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102
Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102
Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42102

Cat. No. GTX42102

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 UCHT1

Isotype

 IgG1

Application

 IHC-Fr, FACS

Reactivity

 Human, Chimpanzee
Package
100 μg ($399)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
FACS 1/50-1/100

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody binds to a region in the ectodomain of human CD3ε and has been shown to bind to a discontinuous epitope near an acidic region of CD3ε opposite the dimer interface.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.

Purification

Protein A purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102

GTX42102 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42102 CD3 antibody [UCHT1] (Low endotoxin, azide free).

Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102

GTX42102 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42102 CD3 antibody [UCHT1] (Low endotoxin, azide free).

Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102

GTX42102 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42102 CD3 antibody [UCHT1] (Low endotoxin, azide free).

Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in IHC (Frozen sections) (IHC-Fr). GTX42102

GTX42102 IHC-Fr Image

IHC-Fr analysis of human tonsil tissue using GTX42102 CD3 antibody [UCHT1] (Low endotoxin, azide free).

Anti-CD3 epsilon antibody [UCHT1] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42102

GTX42102 FACS Image

Figure A. A488 conjugated Mouse anti Human CD38 and RPE-A647 tandem conjugated Mouse IgG1 isotype control. Figure B. A488 conjugated Mouse anti Human CD38 and RPE-Cy5 tandem conjugated Mouse anti Human CD3 (GTX42102). All experiments performed on human Peripheral blood lymphocytes

REFERENCE

Clone Reference

Yarbrough WM et al. Circulation 2003; 108 (14):1753-9 Selective targeting and timing of matrix metalloproteinase inhibition in post-myocardial infarction remodeling.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations