APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:10‐1:50 |
Note :
IHC-P
Recommendations for antigen retrieval : Citrate Buffer pH 6.0 at room temperature for 30 mins.
|
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts with intracytoplasmic portion of the CD3 antigen on the T cells. It stains T cells in cortex as well as medulla of the thymus and lymphoid tissues. This antibody also labels T cell neoplasm, malignant histiocytes and Reed-Sternberg cells in Hodgkin disease.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic human CD3 polypeptide conjugated to bovine serum albumin.
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD3d molecule , CD3-DELTA , IMD19 , T3D
Cellular Localization
Cell membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Database
Research Area
DATA IMAGES
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GTX20828 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX20828 CD3 antibody.
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REFERENCE
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REVIEW
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