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CD3 epsilon antibody [145-2C11]

Cat. No. GTX16181

Reference 10

Host

 Armenian Hamster

Clonality

 Monoclonal

Clone Name

 145-2C11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-Fr, FACS, IP, Activation, Depletion, Neutralizing/Inhibition

Reactivity

 Mouse
Package
250 μg ($319)
See all CD3 epsilon products
PRODUCT

Summary

The 145-2C11 antibody is specific for mouse CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. Such interactions promote T cell receptor signaling (T cell activation) and can result in a number of cellular responses including proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Activation Assay dependent
Depletion Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Tissue / cell preparation, H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3 antigen, epsilon polypeptide , AI504783 , CD3 , CD3epsilon , T3e

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Clone Reference

2018; Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming
Application : ICC/IF, Activation
Reactivity : Mouse
2013; In vivo depletion of leukocytes and platelets following injection of T cell-specific antibodies into mice
Application : Depletion
Reactivity : Mouse
2013; Signaling through C5a receptor and C3a receptor diminishes function of murine natural regulatory T cells
Application : WB, Neutralizing/Inhibition
Reactivity : Mouse
2012; Anti-CD3ε induces splenic B220lo B-cell expansion following anti-CD20 treatment in a mouse model of allosensitization
Application : Neutralizing/Inhibition
2012; NLRC5 deficiency selectively impairs MHC class I- dependent lymphocyte killing by cytotoxic T cells
Application : FACS, Activation
Reactivity : Mouse
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Clone Reference
2018; Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming
Application : ICC/IF, Activation
Reactivity : Mouse
2013; In vivo depletion of leukocytes and platelets following injection of T cell-specific antibodies into mice
Application : Depletion
Reactivity : Mouse
2013; Signaling through C5a receptor and C3a receptor diminishes function of murine natural regulatory T cells
Application : WB, Neutralizing/Inhibition
Reactivity : Mouse
2012; Anti-CD3ε induces splenic B220lo B-cell expansion following anti-CD20 treatment in a mouse model of allosensitization
Application : Neutralizing/Inhibition
2012; NLRC5 deficiency selectively impairs MHC class I- dependent lymphocyte killing by cytotoxic T cells
Application : FACS, Activation
Reactivity : Mouse
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2011; WASp-deficient B cells play a critical, cell-intrinsic role in triggering autoimmunity
Application : IHC-Fr
Reactivity : Mouse
2010; Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas
Application : IP
2008; Statins induce regulatory T cell recruitment via a CCL1 dependent pathway
Application : FACS, Activation
1987; Identification of a monoclonal antibody specific for a murine T3 polypeptide.
Application : IP, Activation
SDS
PBS.pdf
Package List Price ($)
$ 319