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CD3 epsilon antibody [CD3-12]

Anti-CD3 epsilon antibody [CD3-12] used in Flow cytometry (FACS). GTX42110
Anti-CD3 epsilon antibody [CD3-12] used in Western Blot (WB). GTX42110
Anti-CD3 epsilon antibody [CD3-12] used in IHC (Paraffin sections) (IHC-P). GTX42110

Cat. No. GTX42110

Reference 11

Host

 Rat

Clonality

 Monoclonal

Clone Name

 CD3-12

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rabbit, Bovine, Cat, Dog, Chicken, Pig, Alpaca, Amazon Parrot, Bullfrog, Cynomolgus monkey, Duck, Great horned owl (Bubo virginianus), Harbour Porpoise, Horse, Koala, Raccoon, Rhesus Monkey, Sea Lion, Spotted Hyena, Xenopus
Package
25 μg ($219)
See all CD3 epsilon products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/100
IHC-Fr 1/100
FACS 1/50-1/100

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose.

FACS
Membrane permeabilization is required for this application.
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Product Note

This antibody has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence derived from cytoplasmic epitope of CD3 (Glu-Arg-Pro-Pro-Pro-Val-Pro-Asn-Pro-Asp-Tyr-Glu-Pro-Cys) (ERPPPVPNPDYEPC)

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_11178619

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD3e molecule , IMD18 , T3E , TCRE

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 epsilon antibody [CD3-12] used in Flow cytometry (FACS). GTX42110

GTX42110 FACS Image

FACS analysis of red cell lysed human peripheral blood gated on leukocytes using GTX42110 CD3 antibody [CD3-12].
Figure A : RPE conjugated Mouse anti Human CD8 and Alexa Fluor 647 conjugated Rat IgG1 isotype control.
Figure B : RPE conjugated Mouse anti Human CD8 and Alexa Fluor 647 conjugated Rat anti Human CD3 (GTX42110).

Anti-CD3 epsilon antibody [CD3-12] used in Western Blot (WB). GTX42110

GTX42110 WB Image

WB analysis of Jurkat cell lysate using GTX42110 CD3 antibody [CD3-12].

Anti-CD3 epsilon antibody [CD3-12] used in IHC (Paraffin sections) (IHC-P). GTX42110

GTX42110 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX42110 CD3 antibody. Heat mediated antigen retrieval using EDTA buffer pH9.0.

REFERENCE

Application Reference

Huiwen Zheng et al. Cell Rep 2022; 39 (8) : 110864 Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.
Nagatake T et al. Mucosal Immunol 2022; (Epub) Intestinal microbe-dependent ω3 lipid metabolite αKetoA prevents inflammatory diseases in mice and cynomolgus macaques.
Application : IHC-P, IHC-Fr
Reactivity : Mouse
Sugita J et al. Nat Commun 2021; 12 (1) : 1910 Cardiac macrophages prevent sudden death during heart stress.
Application : FACS
Reactivity : Mouse
Sikai Ling et al. Nat Biomed Eng 2021; (Epub) Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice.
Application : ICC/IF
Reactivity : Mouse

Clone Reference

Fadi Jacob et al. bioRxiv 2020; (Epub) Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR-T cell testing.
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REVIEW

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Application Reference
Huiwen Zheng et al. Cell Rep 2022; 39 (8):110864 Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.
Nagatake T et al. Mucosal Immunol 2022; (Epub) Intestinal microbe-dependent ω3 lipid metabolite αKetoA prevents inflammatory diseases in mice and cynomolgus macaques.
Application : IHC-P, IHC-Fr
Reactivity : Mouse
Sugita J et al. Nat Commun 2021; 12 (1):1910 Cardiac macrophages prevent sudden death during heart stress.
Application : FACS
Reactivity : Mouse
Sikai Ling et al. Nat Biomed Eng 2021; (Epub) Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice.
Application : ICC/IF
Reactivity : Mouse
Kirby L et al. Nat Commun 2019; 10 (1):3887 Oligodendrocyte precursor cells present antigen and are cytotoxic targets in inflammatory demyelination.
Application : IHC-Fr
Reactivity : Mouse
Maeda T et al. Int J Cancer 2018; (Epub) Immune-mediated antitumor effect of a transplanted lymph node.
Application : IHC-P
Reactivity : Mouse
Maeda T et al. Lymphat Res Biol 2017; (Epub) Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using * Green and Fluorescein Isothiocyanate-Dextran.
Application : IHC-P
Reactivity : Mouse
Ni G et al. BMC Immunology 2017; (Epub) Blocking IL-10 signalling at the time of immunization does not increase unwanted side effects in mice.
Application : FACS
Reactivity : Mouse
Clone Reference
Fadi Jacob et al. bioRxiv 2020; (Epub) Generation and biobanking of patient-derived glioblastoma organoids and their application in CAR-T cell testing.
Zhang L et al. Mol Cell Probes 2018; 41:43-51 Immunopotentiating effect of Inonotus obliquus fermentation products administered at vaccination in chickens.
Sun F et al. PLoS One 2016; 11 (8):e0160659 Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 219