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CD3 zeta antibody

Anti-CD3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX53919
Anti-CD3 zeta antibody used in Western Blot (WB). GTX53919
Anti-CD3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX53919
Anti-CD3 zeta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53919

Cat. No. GTX53919

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all CD3 zeta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-164 of human CD247 (NP_932170.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD247 molecule , CD3-ZETA , CD3H , CD3Q , CD3Z , IMD25 , T3Z , TCRZ

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX53919

GTX53919 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX53919 CD3 zeta antibody.
Dilution : 1:100

Anti-CD3 zeta antibody used in Western Blot (WB). GTX53919

GTX53919 WB Image

WB analysis of mouse skeletal muscle tissue lysate using GTX53919 CD3 zeta antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD3 zeta antibody used in IHC (Paraffin sections) (IHC-P). GTX53919

GTX53919 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX53919 CD3 zeta antibody.
Dilution : 1:100

Anti-CD3 zeta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53919

GTX53919 ICC/IF Image

ICC/IF analysis of A549 cells using GTX53919 CD3 zeta antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399