GeneTex

  • Country / Location Selection

United States (US)

CD31 antibody [JC70A]

Anti-CD31 antibody [JC70A] used in Flow cytometry (FACS). GTX34495
Anti-CD31 antibody [JC70A] used in Western Blot (WB). GTX34495
Anti-CD31 antibody [JC70A] used in Flow cytometry (FACS). GTX34495
Anti-CD31 antibody [JC70A] used in IHC (Paraffin sections) (IHC-P). GTX34495
Anti-CD31 antibody [JC70A] used in IHC (Paraffin sections) (IHC-P). GTX34495

Cat. No. GTX34495

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 JC70A

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rabbit, Monkey
Package
100 μg ($529)
See all CD31 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Product Note

We do not recommend use of this product for Rat,Pig samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Membrane preparation of a spleen from a patient with hairy cell leukemia

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD31 , CD31/EndoCAM , GPIIA' , PECA1 , PECAM1 , endoCAM , platelet and endothelial cell adhesion molecule 1

Cellular Localization

Cell membrane,Membrane raft,Cell junction

Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-CD31 antibody [JC70A] used in Flow cytometry (FACS). GTX34495

GTX34495 FACS Image

FACS analysis of paraformaldehyde-fixed Jurkat cells using GTX34495 CD31 antibody [JC/70A].
Blue : Primary antibody
Red : Isotype control

Anti-CD31 antibody [JC70A] used in Western Blot (WB). GTX34495

GTX34495 WB Image

WB analysis of THP-1 lysate using GTX34495 CD31 antibody [JC/70A].

Anti-CD31 antibody [JC70A] used in Flow cytometry (FACS). GTX34495

GTX34495 FACS Image

FACS analysis of Jurkat cells using GTX34495 CD31 antibody [JC/70A].
Black : Blank
Grey : Isotype Control
Green : Primary antibody

Anti-CD31 antibody [JC70A] used in IHC (Paraffin sections) (IHC-P). GTX34495

GTX34495 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34495 CD31 antibody [JC/70A].

Anti-CD31 antibody [JC70A] used in IHC (Paraffin sections) (IHC-P). GTX34495

GTX34495 IHC-P Image

IHC-P analysis of human angiosarcoma tissue using GTX34495 CD31 antibody [JC/70A].

REFERENCE

Application Reference

Wang C et al. Biomater Sci 2021; 9 (8) : 3005-3018 CaO2/gelatin oxygen slow-releasing microspheres facilitate tissue engineering efficiency for the osteonecrosis of femoral head by enhancing the angiogenesis and survival of grafted bone marrow mesenchymal stem cells.

Clone Reference

T Miyanaga et al. Cell Mol Biol Lett 2018; 23:49 Angiogenesis after administration of basic fibroblast growth factor induces proliferation and differentiation of mesenchymal stem cells in elastic perichondrium in an in vivo model: mini review of three sequential republication-abridged reports
Application : IHC-P
Reactivity : Rabbit
Tezuka T et al. PLoS One 2015; 10 (3):e0121615 IMD-4690, a novel specific inhibitor for plasminogen activator inhibitor-1, reduces allergic airway remodeling in a mouse model of chronic asthma via regulating angiogenesis and remodeling-related mediators.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for CD31 antibody [JC70A] (GTX34495). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529