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CD32B antibody [C2C3], C-term

Orthogonal
Anti-CD32B antibody [C2C3], C-term used in Western Blot (WB). GTX100262
Anti-CD32B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100262

Cat. No. GTX100262

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD32B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

THP-1

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD32B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240590

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgG receptor IIb , CD32 , CD32B , FCG2 , FCGR2 , FCGR2C , FcRII-c , IGFR2

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD32B antibody [C2C3], C-term used in Western Blot (WB). GTX100262

GTX100262 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD32B antibody [C2C3], C-term (GTX100262) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD32B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100262

GTX100262 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using CD32B(GTX100262) antibody at 1:500 dilution.

REFERENCE

Application Reference

Adhikary T et al. Nucleic Acids Res 2015; 43 (10) : 5033-51 The transcriptional PPARβ/δ network in human macrophages defines a unique agonist-induced activation state.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169