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CD32a antibody [13D7]

Overexpression
Anti-CD32a antibody [13D7] used in Immunoprecipitation (IP). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in Western Blot (WB). GTX84511
Anti-CD32a antibody [13D7] used in Western Blot (WB). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in Western Blot (WB). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511
Anti-CD32a antibody [13D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84511
Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

Cat. No. GTX84511

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 13D7

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all CD32a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
IP 2ug/500ul
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.09 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgG receptor IIa , CD32 , CD32A , CDw32 , FCG2 , FCGR2 , FCGR2A1 , FcGR , IGFR2

Cellular Localization

Cell membrane

Background

Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens.

Database

Research Area

DATA IMAGES

Anti-CD32a antibody [13D7] used in Immunoprecipitation (IP). GTX84511

GTX84511 IP Image

IP analysis of DDDDK tagged CD32a overexpressed HEK293T lysate using GTX84511 CD32a antibody [13D7].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human thyroid tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in Western Blot (WB). GTX84511

GTX84511 WB Image

WB analysis of K562 cell lysate using GTX84511 CD32a antibody [13D7].
Loading : 10 ug per lane
Dilution : 1:200

Anti-CD32a antibody [13D7] used in Western Blot (WB). GTX84511

GTX84511 WB Image

WB analysis of various cell lines using GTX84511 CD32a antibody [13D7].
Loading : 35 ug per lane
Dilution : 1:200

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human breast tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human kidney tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in Western Blot (WB). GTX84511

GTX84511 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CD32a plasmid (Right) or empty vector (Left) for 48 hrs using GTX84511 CD32a antibody [13D7].
Loading : 5 ug per lane

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human breast adenocarcinoma tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human colon adenocarcinoma tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD32a antibody [13D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84511

GTX84511 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD32a plasmid using GTX84511 CD32a antibody [13D7].

Anti-CD32a antibody [13D7] used in IHC (Paraffin sections) (IHC-P). GTX84511

GTX84511 IHC-P Image

IHC-P analysis of human prostate tissue using GTX84511 CD32a antibody [13D7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519