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CD4 antibody [10B5]

Overexpression
Anti-CD4 antibody [10B5] used in Western Blot (WB). GTX84720
Anti-CD4 antibody [10B5] used in Western Blot (WB). GTX84720

Cat. No. GTX84720

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10B5

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($519)
See all CD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD4 (NP_000607) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10727465

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD4 molecule , CD4mut

Cellular Localization

Cell membrane

Background

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

Database

Research Area

DATA IMAGES

Anti-CD4 antibody [10B5] used in Western Blot (WB). GTX84720

GTX84720 WB Image

Overexpression

WB analysis of HEK293T cells transfected with CD4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84720 CD4 antibody [10B5].
Loading : 5 ug per lane

Anti-CD4 antibody [10B5] used in Western Blot (WB). GTX84720

GTX84720 WB Image

WB analysis of various cell lines using GTX84720 CD4 antibody [10B5].
Loading : 35 ug per lane

REFERENCE

Application Reference

Sheu J et al. Research Square 2023; Cocktail therapy of combined SGLT2 inhibitor, entresto and adipose-derived mesenchymal stem cells effectively prevents abdominal aortic aneurysmal associated complication syndrome in rodent
Application : IHC-P
Sgrignoli MR et al. Stem Cell Res 2019; 39 : 101525 Reduction in the inflammatory markers CD4, IL-1, IL-6 and TNF帢 in dogs with keratoconjunctivitis sicca treated topically with mesenchymal stem cells.
Application : IHC-P
Reactivity : Dog
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519