GeneTex
United States (US)

CD40L / CD154 recombinant protein

CD40Ligand-TRAP-recombinant-protein-GTX65393-WB-1_18121409_129.jpg

Cat No. GTX65393

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

ED50 is <0.1 ug/ml determined by its ability to induce TNF-alpha and MIP-1alpha production by murine splenocytes.

Calculated MW

16.4 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers. Stored at -20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd40 Ligand,Cd154,Cd40-L,Cd40L,Higm1,Igm,Imd3,Ly-62,Ly62,T-Bam,Trap,Tnfsf5,Tnlg8B,Gp39,Cd40Lg

Background

CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Research Area

DATA IMAGES
CD40Ligand-TRAP-recombinant-protein-GTX65393-WB-1_18121409_129.jpg

GTX65393 WB Image

Purity of recombinant mouse CD40 Ligand/TRAP (2 µg) was analyzed by reduced SDS-PAGE electrophoresis.

Package List Price ($)
$ 249