GeneTex
United States (US)

CD40L / CD154 recombinant protein

CD40Ligand-TRAP-recombinant-protein-GTX65393-WB-1_18121409_129.jpg

Cat No. GTX65393

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Mouse
Species Mouse
APPLICATION

Application Note

ED50 is <0.1 ug/ml determined by its ability to induce TNF-alpha and MIP-1alpha production by murine splenocytes.

Calculated MW

16.4 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Buffer

Sterile filtered and then lyophilized from 10 mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine

Storage

Reconstitute in water to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers. Stored at -20ºC for future use.

Antigen Species

Mouse

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor necrosis factor ligand superfamily member 5, TRAP, CD40L, IMD3, IGM, TNF-related activation protein, TNFSF5, HIGM1, hCD40L., T cell antigen Gp39, CD40lg, TNFrelated activation protein, TBAM, CD154 antigen, Tumor necrosis factor ligand superfamily member5, CD40-L, sCD40, T-BAM, T BAM, CD40 L, TNF related activation protein

Background

CD40 Ligand (CD40L/TRAP) is a type II transmembrane glycoprotein belonging to the TNF superfamily of ligands. Natually, CD40L is a membrane-bound protein, but proteolytically cleaved 15-18 kDa soluble forms of CD40L are also fully active. CD40L plays an important role in the proliferation and differentiation of B-cells, and is critical for immunoglobulin (Ig) class switching. Recombinant murine CD40 Ligand is a soluble 18 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CDE40L.

Research Area

DATA IMAGES
CD40Ligand-TRAP-recombinant-protein-GTX65393-WB-1_18121409_129.jpg

GTX65393 WB Image

Purity of recombinant mouse CD40 Ligand/TRAP (2 µg) was analyzed by reduced SDS-PAGE electrophoresis.

Package List Price ($)
$ 249