GeneTex
United States (US)

CD41a antibody [HIP8]

Cat No. GTX15021

Host Mouse
Clonality Monoclonal
Clone Name HIP8
Isotype IgG1
Application IHC-Fr, FACS
Reactivity Human
APPLICATION

Application Note

FACS: Tested on human platelets. This can be used at less than or equal to 0.5 mg per 100 ml blood (per 10 6 cells). IHC-Fr: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

113 kDa. ( Note )

Specificity/Sensitivity

GTX145021 reconizes the GPIIb-alpha (heavy chain) of integrin alpha 2B.The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. GTX15021 [HIP8] blocks platelet aggregation andcompletely inhibits ADP, epinephrine, and collagen-induced platelet activation, and partially inhibits ristocetin and thrombin-induced platelet activation. This antibody is useful in the morphological and physiological studies of platelets and megakaryocytes.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length native protein (purified) (Human).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Integrin Subunit Alpha 2B,Bdplt16,Bdplt2,Cd41,Cd41B,Gp2B,Gpiib,Gt,Gta,Hpa3,Ppp1R93,Itga2B

Background

Integrin alpha 2b (CD41) is a calcium-depedent, noncovalently associated heterodimer and contains a heavy chain (GPIIb-alpha) and a light chain (GPIIb-beta) linked by a single disulfied bond.T he integrin alpha 2B chain interacts with the integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major integrin on platelets and is important for platelet adhesion and aggregation.

Database

Research Area

Package List Price ($)
$ 319