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CD44 antibody [Hermes-1]

Anti-CD44 antibody [Hermes-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15883
Anti-CD44 antibody [Hermes-1] used in Flow cytometry (FACS). GTX15883
Anti-CD44 antibody [Hermes-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15883

Cat. No. GTX15883

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 Hermes-1

Isotype

 IgG2a

Application

 ICC/IF, FACS, IP, IHC, Neutralizing/Inhibition

Reactivity

 Human, Mouse, Rabbit, Goat, Sheep, Bovine
Package
100 μg ($399)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:10-1:200
FACS Assay dependent
IP Assay dependent
IHC Assay dependent
Neutralizing/Inhibition Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

80 - 95 kDa lymphocyte surface glycoprotein H-CAM (CD44).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [Hermes-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15883

GTX15883 ICC/IF Image

ICC/IF analysis of MDA-MB-231 cells using GTX15883 CD44 antibody [Hermes-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

Anti-CD44 antibody [Hermes-1] used in Flow cytometry (FACS). GTX15883

GTX15883 FACS Image

FACS analysis of PBMC cells using GTX15883 CD44 antibody [Hermes-1] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4ºC

Anti-CD44 antibody [Hermes-1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15883

GTX15883 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15883 CD44 antibody [Hermes-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

REFERENCE

Application Reference

C Yu et al. Biomed ?Pharmacother 2024; 170 : 116100 Hyaluronan delays human amniotic epithelial stem cell senescence by regulating CD44 isoform switch to activate AKT/mTOR signals
Application : Neutralizing/Inhibition
Li L et al. Am J Transl Res 2021; 13 (10) : 11026-11047 Decellularized extracellular matrix loaded with IPFP-SC for repairing rabbit osteochondral defects.
Application : ICC/IF
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations