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CD75 antibody [N2C3]

Anti-CD75 antibody [N2C3] used in Western Blot (WB). GTX104018
Anti-CD75 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018
Anti-CD75 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018

Cat No. GTX104018

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

CD75 tansfected 293T cell

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD75. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC48859 antibody, SIAT1 antibody, ST6GalI antibody, ST6N antibody, ST6GAL1 antibody, "CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1 antibody", "CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase antibody", ST6Gal I antibody, B-cell antigen CD75 antibody, "alpha 2,6-ST 1 antibody", "sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase) antibody", "beta-galactoside alpha-2,6-sialyltransferase 1 antibody", "ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 antibody"

Cellular Localization

Golgi apparatus , Golgi stack membrane; Single-pass type II membrane protein , Secreted

Background

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]
DATA IMAGES
Anti-CD75 antibody [N2C3] used in Western Blot (WB). GTX104018

GTX104018 WB Image

Western Blot analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody.
A: Non-transfected lysate.
B: ST6GAL1 transfected lysate.
12% SDS PAGE
GTX104018 diluted at 1:500

Anti-CD75 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018

GTX104018 IHC-P Image

CD75 antibody [N2C3] detects CD75 protein at Golgi apparatus in human hepatocellular carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
CD75 antibody [N2C3] (GTX104018) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD75 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104018

GTX104018 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using CD75(GTX104018) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
There are currently no references for CD75 antibody [N2C3] (GTX104018). Be the first to share your publications with this product.
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169