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CD82 antibody

Anti-CD82 antibody used in Western Blot (WB). GTX31310
Anti-CD82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31310
Anti-CD82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31310

Cat. No. GTX31310

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all CD82 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

A549 Cell Lysate(GTX27910) , Human Colon Tissue Lysate(GTX27928)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CD82 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human CD82.The immunogen is located within the last 50 amino acids of CD82.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD82 molecule , 4F9 , C33 , GR15 , IA4 , KAI1 , R2 , SAR2 , ST6 , TSPAN27

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD82 antibody used in Western Blot (WB). GTX31310

GTX31310 WB Image

WB analysis of A549 cell lysate using GTX31310 CD82 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Anti-CD82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31310

GTX31310 IHC-P Image

IHC-P analysis of human colon tissue using GTX31310 CD82 antibody.
Working concentration : 2.5 μg/ml

Anti-CD82 antibody used in IHC (Paraffin sections) (IHC-P). GTX31310

GTX31310 IHC-P Image

IHC-P analysis of human colon tissue using GTX31310 CD82 antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399