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CDKN2A / p14ARF antibody

KO/KD Orthogonal
Anti-CDKN2A / p14ARF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129903
Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX129903
Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX129903
Anti-CDKN2A / p14ARF antibody used in Immunoprecipitation (IP). GTX129903
Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX129903

Cat. No. GTX129903

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CDKN2A / p14ARF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CDKN2A / p14ARF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886119

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin-dependent kinase inhibitor 2A Antibody , P16INK4 Antibody , TP16 Antibody , CDKN2 Antibody , MTS1 Antibody , P16 Antibody , P14ARF Antibody , CDKN2A Antibody , CMM2 Antibody , P16INK4A Antibody , MLM Antibody , ARF Antibody , INK4 Antibody , P19ARF Antibody , P19 Antibody , INK4A Antibody , P16-INK4A Antibody , CDK4I Antibody , P14 Antibody , MTS-1 Antibody

Background

This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, MDM1, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-CDKN2A / p14ARF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129903

GTX129903 ICC/IF Image

p14ARF antibody detects p14ARF protein at nucleolus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p14ARF protein stained by p14ARF antibody (GTX129903) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX129903

GTX129903 WB Image

KO/KD

Wild-type (WT) and p14ARF knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody (GTX129903) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX129903

GTX129903 WB Image

p14ARF antibody detects p14ARF protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with p14ARF antibody (GTX129903) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CDKN2A / p14ARF antibody used in Immunoprecipitation (IP). GTX129903

GTX129903 IP Image

Immunoprecipitation of p14ARF protein from HeLa whole cell extracts using 5 μg of p14ARF antibody (GTX129903).
Western blot analysis was performed using p14ARF antibody (GTX129903).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-CDKN2A / p14ARF antibody used in Western Blot (WB). GTX129903

GTX129903 WB Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2020.PMID: 31920463

REFERENCE

Application Reference

Yang Y et al. Cancer Cell Int 2020; 20 : 9 Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169