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CENPH antibody, Internal

Anti-CENPH antibody, Internal used in Western Blot (WB). GTX80491
Anti-CENPH antibody, Internal used in Flow cytometry (FACS). GTX80491
Anti-CENPH antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX80491

Cat. No. GTX80491

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all CENPH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 136-162 amino acids from the Central region of human CENPH.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

centromere protein H

Cellular Localization

Nucleus

Background

Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CENPH antibody, Internal used in Western Blot (WB). GTX80491

GTX80491 WB Image

WB analysis of HeLa cell lysate (35ug/lane) using GTX80491 CENPH antibody, Internal.

Anti-CENPH antibody, Internal used in Flow cytometry (FACS). GTX80491

GTX80491 FACS Image

FACS analysis of HeLa cells using GTX80491 CENPH antibody, Internal.
Top histogram : negative control
Bottom histogram : HeLa cells

Anti-CENPH antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX80491

GTX80491 IHC-P Image

IHC-P analysis of human testis tissue using GTX80491 CENPH antibody, Internal.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399