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CITED2 antibody

Anti-CITED2 antibody used in Western Blot (WB). GTX129265
Anti-CITED2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129265

Cat. No. GTX129265

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CITED2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

293T , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CITED2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885946

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 , ASD8 , MRG-1 , MRG1 , P35SRJ , VSD2

Cellular Localization

Nucleus

Background

The protein encoded by this gene inhibits transactivation of HIF1A-induced genes by competing with binding of HIF1a to p300-CH1. Mutations in this gene are a cause of cardiac septal defects. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CITED2 antibody used in Western Blot (WB). GTX129265

GTX129265 WB Image

CITED2 antibody detects CITED2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
CITED2 antibody (GTX129265) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CITED2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129265

GTX129265 ICC/IF Image

CITED2 antibody detects CITED2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CITED2 protein stained by CITED2 antibody (GTX129265) diluted at 1:1000.
Blue: Hoechst 33342 staining.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169