COL17A1 antibody [NC16a-3]

Cat. No. GTX54647

Host	Mouse
Clonality	Monoclonal
Clone Name	NC16a-3
Isotype	IgG1
Application	WB, IHC-P, IHC-Fr, ELISA
Reactivity	Human
Package	250 μg ($599)

See all COL17A1 products

Datasheet
Document

View product citations for antibody GTX54647 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
IHC-P	1:40
IHC-Fr	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

150 kDa. ( Note )

Product Note

This antibody recognizes the NC16a-domain of collagen XVII (aa 545-557). It does not cross-react with mouse and rat skin based on internal testing; other species not tested.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant NC16a-domain of collagen XVII. The epitope has been mapped to aa545-557 (PMID: 19158842).

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type XVII alpha 1 chain , BA16H23.2 , BP180 , BPA-2 , BPAG2 , ERED , LAD-1

Background

This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1308 COL17A1
UniProt: Q9UMD9 COL17A1

Research Area

DATA IMAGES

GTX54647 IHC-Fr Image

IHC-Fr analysis of normal human skin (left) and skin tissue in absence of collagenXVII (middle) from patients with inherited skin-blistering disorders using GTX54647 COL17A1 antibody [NC16a-3]. Another skin tissue in absence of laminin 5 (right) was stained with a rabbit anti-laminin 5 antibody.

GTX54647 WB Image

WB analysis of keratinocyte extract (E) and keratinocyte medium (M) using GTX54647 COL17A1 antibody [NC16a-3]. This antibody detects collagen XVII and its 120kDa shed ectodomain.

REFERENCE

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REVIEW

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SDS
PBS.pdf

Package

List Price ($)

250 μg

$ 599

Qty

COL17A1 antibody [NC16a-3]

Cat. No. GTX54647

Host

Clonality

Clone Name

Isotype

Application

Reactivity