COL1A1 antibody [N1N2], N-term

Name: COL1A1 antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX112731
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-COL1A1 antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX112731

Anti-COL1A1 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112731

Anti-COL1A1 antibody [N1N2], N-term used in Western Blot (WB). GTX112731

Anti-COL1A1 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112731

Cat. No. GTX112731

Reference 21

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity	Polyodon spathula, Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all COL1A1 products

View product citations for antibody GTX112731 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
IP	1:100-1:500

Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human COL1A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721155

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Gene ID: 1277 COL1A1
UniProt: P02452 COL1A1

Research Area

DATA IMAGES

GTX112731 IP Image

Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 μg of COL1A1 antibody [N1N2], N-term (GTX112731).
Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX112731 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX112731 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

GTX112731 WB Image

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112731 WB Image

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112731 ICC/IF Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at vesicle by immunofluorescent analysis.
Sample: NIH-3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX112731 ICC/IF Image

COL1A1 antibody [N1N2], N-term detects COL1A1 protein at vesicle by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX112731 IHC-P Image

COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min