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COL4A1 antibody

Anti-COL4A1 antibody used in Western Blot (WB). GTX130215
Anti-COL4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130215
Anti-COL4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130215
Anti-COL4A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130215

Cat. No. GTX130215

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COL4A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

161 kDa. ( Note )

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus of human COL4A1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886210

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type IV alpha 1 chain , BSVD , RATOR

Cellular Localization

Secreted , extracellular space , extracellular matrix , basement membrane

Background

This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COL4A1 antibody used in Western Blot (WB). GTX130215

GTX130215 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody (GTX130215) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COL4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130215

GTX130215 IHC-P Image

COL4A1 antibody detects COL4A1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
COL4A1 antibody (GTX130215) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX130215

GTX130215 IHC-P Image

COL4A1 antibody detects COL4A1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
COL4A1 stained by COL4A1 antibody (GTX130215) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COL4A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130215

GTX130215 ICC/IF Image

COL4A1 antibody detects COL4A1 protein at vesicle by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL4A1 stained by COL4A1 antibody (GTX130215) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Hui L et al. PLoS Pathog 2020; 16 (4) : e1008509 Matrix metalloproteinase 9 facilitates Zika virus invasion of the testis by modulating the integrity of the blood-testis barrier.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169