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COMT antibody

KO/KD
Anti-COMT antibody used in Western Blot (WB). GTX101233
Anti-COMT antibody used in Western Blot (WB). GTX101233
Anti-COMT antibody used in Western Blot (WB). GTX101233
Anti-COMT antibody used in IHC (Paraffin sections) (IHC-P). GTX101233
Anti-COMT antibody used in ELISA (ELISA). GTX101233

Cat. No. GTX101233

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, Sandwich ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all COMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX101233 , Detection: GTX89277
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

mouse spleen , Molt-4 , 293T

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human COMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950015

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catechol-O-methyltransferase , HEL-S-98n

Cellular Localization

Isoform Soluble: Cytoplasm , Isoform Membrane-bound: Cell membrane

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COMT antibody used in Western Blot (WB). GTX101233

GTX101233 WB Image

KO/KD

Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody (GTX101233) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COMT antibody used in Western Blot (WB). GTX101233

GTX101233 WB Image

COMT antibody detects COMT protein by Western blot analysis.
A. 50 μg mouse spleen lysate/extract
12 % SDS-PAGE
COMT antibody (GTX101233) dilution: 1:500

Anti-COMT antibody used in Western Blot (WB). GTX101233

GTX101233 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX101233 diluted at 1:1000
Anti-COMT antibody used in IHC (Paraffin sections) (IHC-P). GTX101233

GTX101233 IHC-P Image

COMT antibody detects COMT protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
COMT antibody (GTX101233) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COMT antibody used in ELISA (ELISA). GTX101233

GTX101233 ELISA Image

ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 μg/mL and GTX89277 for detection at a concentration of 0.5 μg/mL.

REFERENCE

Application Reference

Yoon JH et al. Gastric Cancer 2018; (Epub) Gastrokine 1 protein is a potential theragnostic target for gastric cancer.
Application : WB
Reactivity : Human
Ikeda M et al. Molecules 2017; 22 (8) Inhibitory Effect of Bovine Lactoferrin on Catechol-O-Methyltransferase.
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169