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CPSF6 antibody

Anti-CPSF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX115537
Anti-CPSF6 antibody used in Western Blot (WB). GTX115537
Anti-CPSF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX115537
Anti-CPSF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115537

Cat No. GTX115537

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CPSF6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cleavage and polyadenylation specific factor 6 , CFIM , CFIM68 , CFIM72 , HPBRII-4 , HPBRII-7

Cellular Localization

Nucleus

Background

The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. The cleavage factor complex is composed of four polypeptides. This gene encodes the 68kD subunit. It has a domain organization reminiscent of spliceosomal proteins. [provided by RefSeq]

Database

DATA IMAGES
Anti-CPSF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX115537

GTX115537 IHC-P Image

CPSF6 antibody detects CPSF6 protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
CPSF6 antibody (GTX115537) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CPSF6 antibody used in Western Blot (WB). GTX115537

GTX115537 WB Image

CPSF6 antibody detects CPSF6 protein by Western blot analysis.
A. 30 µg 293T
B. 30 µg A431
C. 30 µg HeLa
D. 30 µg HepG2
7.5 % SDS-PAGE
CPSF6 antibody (GTX115537) dilution: 1:5000

Anti-CPSF6 antibody used in IHC (Paraffin sections) (IHC-P). GTX115537

GTX115537 IHC-P Image

CPSF6 antibody detects CPSF6 protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CPSF6 antibody (GTX115537) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CPSF6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115537

GTX115537 ICC/IF Image

CPSF6 antibody detects CPSF6 protein at nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CPSF6 protein stained by CPSF6 antibody (GTX115537) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169