GeneTex

  • Country / Location Selection

United States (US)

CREM antibody

Anti-CREM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114146
Anti-CREM antibody used in Western Blot (WB). GTX114146
Anti-CREM antibody used in Western Blot (WB). GTX114146
Anti-CREM antibody used in Western Blot (WB). GTX114146

Cat. No. GTX114146

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CREM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Neuro2A , GL261 , PC-12 , Rat2 , NT2D1

Predict Reactivity

Bovine, Dog, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CREM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623611

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element modulator , CREM-2 , ICER , hCREM-2

Cellular Localization

Nucleus

Background

This gene encodes a bZIP transcription factor that binds to the cAMP responsive element found in many viral and cellular promoters. It is an important component of cAMP-mediated signal transduction during the spermatogenetic cycle, as well as other complex processes. Alternative promoter and translation initiation site usage allows this gene to exert spatial and temporal specificity to cAMP responsiveness. Multiple alternatively spliced transcript variants encoding several different isoforms have been found for this gene, with some of them functioning as activators and some as repressors of transcription. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CREM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114146

GTX114146 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CREM(GTX114146) antibody at 1:200 dilution.

Anti-CREM antibody used in Western Blot (WB). GTX114146

GTX114146 WB Image

CREM antibody detects CREM protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
12 % SDS-PAGE
CREM antibody (GTX114146) dilution: 1:1000

Anti-CREM antibody used in Western Blot (WB). GTX114146

GTX114146 WB Image

CREM antibody detects CREM protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12 % SDS-PAGE
CREM antibody (GTX114146) dilution: 1:1000

Anti-CREM antibody used in Western Blot (WB). GTX114146

GTX114146 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
12% SDS PAGE
CREM antibody
GTX114146 diluted at 1:1000

REFERENCE

There are currently no references for CREM antibody (GTX114146). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CREM antibody (GTX114146). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169