GeneTex

  • Country / Location Selection

United States (US)

CRHR1 antibody

Orthogonal
Anti-CRHR1 antibody used in Western Blot (WB). GTX130244
Anti-CRHR1 antibody used in Western Blot (WB). GTX130244
Anti-CRHR1 antibody used in Western Blot (WB). GTX130244
Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

Cat. No. GTX130244

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CRHR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

rat brain , mouse brain

Predict Reactivity

Rabbit, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CRHR1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

corticotropin releasing hormone receptor 1 , CRF-R , CRF-R-1 , CRF-R1 , CRF1 , CRFR-1 , CRFR1 , CRH-R-1 , CRH-R1 , CRHR , CRHR1L

Cellular Localization

Cell membrane

Background

This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants.

Database

Research Area

DATA IMAGES

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

CRHR1 antibody detects CRHR1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CRHR1 antibody (GTX130244) diluted at 1:1000.

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

U87-MG whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CRHR1 antibody (GTX130244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

CRHR1 antibody detects CRHR1 protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CRHR1 antibody (GTX130244) diluted at 1:1000.

Anti-CRHR1 antibody used in Western Blot (WB). GTX130244

GTX130244 WB Image

Orthogonal

The data was published in the journal Transl Psychiatry in 2018. PMID: 29780165

REFERENCE

Application Reference

Koyama M et al. Anticancer Res 2021; 41 (9) : 4259-4269 Gelatin Sponge as an Anchorage for Three-dimensional Culture of Colorectal Cancer Cells.
Application : IHC-P
Reactivity : Human
Kai Y et al. Transl Psychiatry 2018; 8 (1) : 100 A medial prefrontal cortex-nucleus acumens corticotropin-releasing factor circuitry for neuropathic pain-increased susceptibility to opioid reward.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for CRHR1 antibody (GTX130244). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169