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CRK antibody [3G11E8]

Anti-CRK antibody [3G11E8] used in Western Blot (WB). GTX60488
Anti-CRK antibody [3G11E8] used in Flow cytometry (FACS). GTX60488
Anti-CRK antibody [3G11E8] used in ELISA (ELISA). GTX60488
Anti-CRK antibody [3G11E8] used in IHC (Paraffin sections) (IHC-P). GTX60488
Anti-CRK antibody [3G11E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60488
Anti-CRK antibody [3G11E8] used in Western Blot (WB). GTX60488
Anti-CRK antibody [3G11E8] used in IHC (Paraffin sections) (IHC-P). GTX60488

Cat. No. GTX60488

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3G11E8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($409)
See all CRK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CRK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CRK proto-oncogene, adaptor protein , CRKII , p38

Cellular Localization

Cytoplasm,Cell membrane

Background

This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CRK antibody [3G11E8] used in Western Blot (WB). GTX60488

GTX60488 WB Image

WB analysis of HEK293 (1) and CRK(AA: 1-204)-hIgGFc transfected HEK293 (2) cell lysate using GTX60488 CRK antibody [3G11E8].

Anti-CRK antibody [3G11E8] used in Flow cytometry (FACS). GTX60488

GTX60488 FACS Image

FACS analysis of HeLa cells using GTX60488 CRK antibody [3G11E8].
Blue : CRK
Red : negative control

Anti-CRK antibody [3G11E8] used in ELISA (ELISA). GTX60488

GTX60488 ELISA Image

ELISA analysis of antigen using GTX60488 CRK antibody [3G11E8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-CRK antibody [3G11E8] used in IHC (Paraffin sections) (IHC-P). GTX60488

GTX60488 IHC-P Image

IHC-P analysis of lung cancer tissue using GTX60488 CRK antibody [3G11E8].

Anti-CRK antibody [3G11E8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60488

GTX60488 ICC/IF Image

ICC/IF analysis of 3T3-L1 cells using GTX60488 CRK antibody [3G11E8].
Green : CRK
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-CRK antibody [3G11E8] used in Western Blot (WB). GTX60488

GTX60488 WB Image

WB analysis of human CRK (AA: 1-204) recombinant protein using GTX60488 CRK antibody [3G11E8].

Anti-CRK antibody [3G11E8] used in IHC (Paraffin sections) (IHC-P). GTX60488

GTX60488 IHC-P Image

IHC-P analysis of intima cancer tissue using GTX60488 CRK antibody [3G11E8].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409