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CRMP2 antibody [N1N3]

Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420
Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420
Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420
Anti-CRMP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113420
Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420

Cat. No. GTX113420

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CRMP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:100000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

mouse brain , rat brain , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human CRMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036670

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydropyrimidinase like 2 , CRMP-2 , CRMP2 , DHPRP2 , DRP-2 , DRP2 , N2A3 , ULIP-2 , ULIP2

Cellular Localization

Cytoplasm

Background

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

Database

Research Area

DATA IMAGES

Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420

GTX113420 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] (GTX113420) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420

GTX113420 IHC-P Image

CRMP2 antibody [N1N3] detects CRMP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CRMP2 stained by CRMP2 antibody [N1N3] (GTX113420) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CRMP2 antibody [N1N3] used in Western Blot (WB). GTX113420

GTX113420 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP2 antibody [N1N3] (GTX113420) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CRMP2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113420

GTX113420 ICC/IF Image

CRMP2 antibody [N1N3] detects CRMP2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CRMP2 stained by CRMP2 antibody [N1N3] (GTX113420) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CRMP2 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX113420

GTX113420 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using CRMP2(GTX113420) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Yang L et al. Front Pharmacol 2021; 12 : 763181 Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Trillium tschonoskii Maxim. Treatment in a Rat Model of Focal Ischemia.
Agrawal L et al. Neuropharmacology 2019; 158 : 107712 Role of serotonin 4 receptor in the growth of hippocampal neurons during the embryonic development in mice.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169