APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.55 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human CSRP3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950051
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cysteine and glycine rich protein 3 , CLP , CMD1M , CMH12 , CRP3 , LMO4 , MLP
Cellular Localization
Nucleus , Cytoplasm , cytoskeleton
Background
This gene encodes a member of the CSRP family of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this protein is found in a group of proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Mutations in this gene are thought to cause heritable forms of hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM) in humans. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX110536 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody (GTX110536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110536 WB Image
Human heart (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CSRP3 antibody (GTX110536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX110536 IHC-P Image
CSRP3 antibody detects CSRP3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse muscle.CSRP3 stained by CSRP3 antibody (GTX110536) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX110536 IHC-P Image
CSRP3 antibody detects CSRP3 protein at cytosol on C2C12 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded C2C12 xenograft.
CSRP3 antibody (GTX110536) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX110536 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using CSRP3(GTX110536) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
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GTX110536 IHC-P Image
CSRP3 antibody detects CSRP3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.CSRP3 stained by CSRP3 antibody (GTX110536) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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