GeneTex
United States (US)

CTGF antibody

GTX124232_43166_20180615_WB_B_18121410_287.jpg
GTX124232_40898_WB_R_Bladder_18121410_418.jpg
GTX124232_41794_20141017_WB_18121410_605.jpg
GTX124232_43166_20180615_WB_Fraction_18121410_150.jpg
GTX124232_40898_IHC_18121410_964.jpg
GTX124232_41577_WB_18121410_334.jpg
GTX124232_40653_WB_M_Heart_18121410_364.jpg

Cat No. GTX124232

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat, Bovine, Baboon
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , HUVEC , Mouse heart , Rat bladder , CTGF-transfected 293T , HepG2 , HepG2 conditioned medium

Predict Reactivity

Sheep, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTGF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Connective Tissue Growth Factor,Ccn2,Hcs24,Igfbp8,Nov2,Ctgf

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX124232_43166_20180615_WB_B_18121410_287.jpg

GTX124232 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124232) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124232_40898_WB_R_Bladder_18121410_418.jpg

GTX124232 WB Image

Sample (1 µg of whole cell lysate)
A: Rat bladder
10% SDS PAGE
GTX124232 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124232_41794_20141017_WB_18121410_605.jpg

GTX124232 WB Image

CTGF antibody detects CTGF protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124232) diluted by 1:1000.

GTX124232_43166_20180615_WB_Fraction_18121410_150.jpg

GTX124232 WB Image

HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124232_40898_IHC_18121410_964.jpg

GTX124232 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using CTGF(GTX124232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX124232_41577_WB_18121410_334.jpg

GTX124232 WB Image

CTGF antibody detects CTGF protein by Western blot analysis.
A. 30 μg HUVEC whole cell lysate/extract
12 % SDS-PAGE
CTGF antibody (GTX124232) dilution: 1:5000

GTX124232_40653_WB_M_Heart_18121410_364.jpg

GTX124232 WB Image

Sample (50 µg of whole cell lysate)
A: mouse heart
10% SDS PAGE
GTX124232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

CTGF antibody Cat No. GTX124232

Rating ( Average 4.3 based on 3 users reviews)
Application
Western Blot(WB) ( Average 4.3 based on 3 users reviews)
Date : Anonymous submitted on 17-Aug-2012
Score :
Application Tested : WB
Sample Species : Ms
Sample : liver tissue
Amount used : 50μg
Blocking : Odyssey Blocking Buffer (No Tween), 25°C, 1Hr
Primary Antibody : 1μg/ml, 4°C, overnightHr
Date : Anonymous submitted on 23-Dec-2011
Score :
Application Tested : WB
Sample Species : Ms
Sample : small intestine tissue
Amount used : 40μg
Primary Antibody : 1:500, 4°C, overnightHr
Date : Anonymous submitted on 07-Nov-2011
Score :
Application Tested : WB
Sample : CCD18co cells mouse intestine tissue
Amount used : 20μg
Blocking : 5% non fat milk, 25°C, 1Hr
Primary Antibody : 1:500, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169