APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HUVEC , Rat bladder , CTGF-transfected 293T
Predict Reactivity
Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.65 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human CTGF. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11174939
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cellular communication network factor 2 , CTGF , HCS24 , IGFBP8 , NOV2
Cellular Localization
Secreted , extracellular space , extracellular matrix
Background
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX124233 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX124233 WB Image
HepG2 conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX124233 WB Image
Sample (50 ug of whole cell lysate)
A: Rat bladder
10% SDS PAGE
GTX124233 diluted at 1:500
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GTX124233 IHC-P Image
CTGF antibody detects secreted CTGF protein in mouse placenta by immunohistochemical analysis.
Sample: Paraffin-embedded mouse placenta.
CTGF antibody (GTX124233) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX124233 WB Image
Sample (30 ug of whole cell lysate)
A: HUVEC
10% SDS PAGE
GTX124233 diluted at 1:1000
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REFERENCE
REVIEW
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