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CTNNAL1 antibody [N3C2], Internal

Anti-CTNNAL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112226
Anti-CTNNAL1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112226
Anti-CTNNAL1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112226
Anti-CTNNAL1 antibody [N3C2], Internal used in Western Blot (WB). GTX112226

Cat. No. GTX112226

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CTNNAL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

HeLa , HepG2

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CTNNAL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950060

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

catenin alpha like 1 , ACRP , CLLP , alpha-CATU

Cellular Localization

Cytoplasm , cytoskeleton , Cell membrane

Background

May modulate the Rho pathway signaling by providing a scaffold for the Lbc Rho guanine nucleotide exchange factor (ARHGEF1).

Database

Research Area

DATA IMAGES

Anti-CTNNAL1 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112226

GTX112226 ICC/IF Image

Alpha-catulin antibody [N3C2], Internal detects Alpha-catulin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Alpha-catulin protein stained by Alpha-catulin antibody [N3C2], Internal (GTX112226) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CTNNAL1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112226

GTX112226 IHC-P Image

Alpha-catulin antibody [N3C2], Internal detects Alpha-catulin protein at cytoplasm on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
Alpha-catulin antibody [N3C2], Internal (GTX112226) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CTNNAL1 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX112226

GTX112226 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-catulin(GTX112226) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CTNNAL1 antibody [N3C2], Internal used in Western Blot (WB). GTX112226

GTX112226 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX112226 diluted at 1:1000

REFERENCE

Application Reference

Gingras J et al. J Cell Sci 2016; 129 (5) : 898-911 α-Dystrobrevin-1 recruits Grb2 and α-catulin to organize neurotransmitter receptors at the neuromuscular junction.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169