APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
| 2 μg/ml |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.08 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human IL-8
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
C-X-C motif chemokine ligand 8 , GCP-1 , GCP1 , IL8 , LECT , LUCT , LYNAP , MDNCF , MONAP , NAF , NAP-1 , NAP1
Cellular Localization
Secreted
Background
The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
![Anti-CXCL8 / IL8 antibody [3IL8-H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15763](/templates/default/js/lightbox/images/loading.gif "Anti-CXCL8 / IL8 antibody [3IL8-H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15763")
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GTX15763 ICC/IF Image
ICC/IF analysis of U-87 MG cells treated 1 uM thapsigargin for 24 hours using GTX15763 CXCL8 / IL8 antibody [3IL8-H10]. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
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GTX15763 ELISA Image
Sandwich ELISA analysis of serial diluted human IL-8 recombinant protein(50ul per well) using GTX15763 CXCL8 / IL8 antibody [3IL8-H10] (capture antibody) at 3 μg/mL and biotinylated mouse anti-human IL-8 monoclonal antibody (detection antibody) at 0.25 μg/mL.
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REFERENCE
There are currently no references for CXCL8 / IL8 antibody [3IL8-H10] (GTX15763). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CXCL8 / IL8 antibody [3IL8-H10] (GTX15763). Be the first to share your experience with this product.
![Anti-CXCL8 / IL8 antibody [3IL8-H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15763](/upload/website/prouct_img/small/GTX15763/GTX15763_298_ICC-IF_w_23060620_274.webp "Anti-CXCL8 / IL8 antibody [3IL8-H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15763")
![Anti-CXCL8 / IL8 antibody [3IL8-H10] used in ELISA (ELISA). GTX15763](/upload/website/prouct_img/small/GTX15763/GTX15763_65_ELISA_w_23060620_916.webp "Anti-CXCL8 / IL8 antibody [3IL8-H10] used in ELISA (ELISA). GTX15763")
![Anti-CXCL8 / IL8 antibody [3IL8-H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15763](/upload/website/prouct_img/normal/GTX15763/GTX15763_298_ICC-IF_w_23060620_274.webp "Anti-CXCL8 / IL8 antibody [3IL8-H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15763")
![Anti-CXCL8 / IL8 antibody [3IL8-H10] used in ELISA (ELISA). GTX15763](/upload/website/prouct_img/normal/GTX15763/GTX15763_65_ELISA_w_23060620_916.webp "Anti-CXCL8 / IL8 antibody [3IL8-H10] used in ELISA (ELISA). GTX15763")